Toyo Seikan Group has patented a culture container for lymphocytes with immobilized and non-immobilized surfaces. The container is made of gas-permeable film, with anti-CD3 antibodies immobilized on one surface. The patent claims a specific concentration of antibodies and gas volume for optimal cell culture. GlobalData’s report on Toyo Seikan Group gives a 360-degree view of the company including its patenting strategy. Buy the report here.

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According to GlobalData’s company profile on Toyo Seikan Group, Fuel cell powered drones was a key innovation area identified from patents. Toyo Seikan Group's grant share as of January 2024 was 33%. Grant share is based on the ratio of number of grants to total number of patents.

Culture bag for lymphocyte culturing with immobilized and non-immobilized surfaces

Source: United States Patent and Trademark Office (USPTO). Credit: Toyo Seikan Group Holdings Ltd

A recently granted patent (Publication Number: US11884907B2) discloses a novel culture bag designed specifically for culturing lymphocytes. The bag comprises two film members, each with the necessary gas permeability for cell culture, formed into a bag shape. The first film member's interior surface is an immobilized activating surface achieved by immobilizing anti-CD3 antibodies at a concentration ranging from 10 to 300 ng/cm2. In contrast, the second film member's interior surface serves as a non-immobilized proliferating surface without the anti-CD3 antibodies. Additionally, the culture bag is sealed with a specific amount of gas, ranging from 0.01 to 4 ml per cm2 of the bottom surface area.

This innovative culture bag design offers a unique solution for culturing lymphocytes, providing distinct surfaces for activation and proliferation within the same bag. By immobilizing anti-CD3 antibodies on one surface and leaving the other surface non-immobilized, the bag allows for controlled and efficient cell culture. The precise sealing of gas within the bag further enhances the culturing process, ensuring optimal conditions for lymphocyte growth and maintenance. Overall, this patented culture bag represents a significant advancement in the field of cell culture technology, offering a specialized tool for researchers and professionals working with lymphocytes.

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GlobalData Patent Analytics tracks bibliographic data, legal events data, point in time patent ownerships, and backward and forward citations from global patenting offices. Textual analysis and official patent classifications are used to group patents into key thematic areas and link them to specific companies across the world’s largest industries.